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Perentie - Tropical Rock Mini-Exhibit - 2 animals. Freshwater Crocodile - Arid Pool Mini-Exhibit - 2 animals. Australian Masked Owl - Bush Grove Mini-Exhibit - 2 animals. Eastern Grey Kangaroo - Outback Exhibit - 4 animals. Black Falcon - Bush Grove Mini-Exhibit - 2 animals.
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Southern South American Puma - Tropical Exhibit - 2 animals. Outback Animal Reserve Unobtainable Star Animal: Olympic Black Bear - Temperate Exhibit - 2 animals - obtained from East Coast City Zoo. There are twenty challenge locations all up, detailed below (the number before each animal denotes level that the animal becomes available): It's annoying because there's no sorting feature in the reserve, but saves a ton of money in the next zoo when you can just select your animals from there without any adoption costs. When you finish a challenge zoo, send ALL your animals from that zoo to the reserve (releasing level 15s as you go). Another quirk of challenge mode is that it does NOT make traditional save states-the game automatically saves any progress you make in an area unless you clear that save information. For the most efficient play-through order, see the Checklist on Challenge Mode below. In each zoo, there is one Star Species that MUST be transferred from a particular different zoo location by sending that animal to the reserve (the option next to sell in the animal's menu).
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The zoos don't always feature the same species, so you'll have to play multiple zones and transfer animals from one zoo to another to fill out your collections.